The image segmentation technique and associated tools are commonly used to produce anatomical measurements of lesions or structures. The progressive development of medical imaging techniques has made it possible for an increased number of structures to be assessed and correlated with the morphological alterations associated with disease or treatment. This study was the first to demonstrate the step-by-step application of 3D Slicer, a user-friendly and easily accessible open-source multiplatform software program for the segmentation and volume estimation of the pulp spaces of teeth treated with REPs. The open-source software (3D Slicer) was found to be as accurate as the commercially available software with regard to the volumetric assessment of the post-REP pulp space. The mean decreases in the pulp space volumes measured using OsiriX MD and 3D Slicer were 25.06% ± 19.45% and 26.10% ± 18.90%, respectively. The pulp space volumes measured using both programs revealed a statistically significant decrease in the pulp space volume following the REP (P 0.05). The data were statistically analyzed using t-tests and paired t-tests (P = 0.05). The volumetric changes in the teeth’s pulp spaces were assessed using semi-automated techniques in both programs. For the subsequent clinical application, pre- and post-procedure cone beam computed tomography scans of 35 immature permanent teeth with necrotic pulp and periradicular pathosis that had been treated with a cell-homing concept-based REP were processed using the two biomedical DICOM software programs (OsiriX MD and 3D Slicer). An Invitro assessment was performed to check the reliability and sensitivity of the two medical imaging programs in use. This study sought to compare the accuracy of two different artificial intelligence-based medical imaging programs namely OsiriX MD (v 9.0, Pixmeo SARL, Bernex Switzerland, ) and 3D Slicer ( ), in terms of estimating the volume of the pulp space following a REP. However, to date, only a few studies have investigated the accuracy of the available domain-specialized medical imaging tools with regard to three-dimensional (3D) volumetric assessment. The volumetric change that occurs in the pulp space over time represents a critical measure when it comes to determining the secondary outcomes of regenerative endodontic procedures (REPs).

Osirix lite segmentation